TF-Test Quantified: a new technique for diagnosis of Schistosoma mansoni eggs

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorSantos, Bianca Martins-
Autor(es): dc.creatorSoares, Felipe Augusto-
Autor(es): dc.creatorRosa, Stefany Laryssa-
Autor(es): dc.creatorFerreira Gomes, Denise da Conceicao-
Autor(es): dc.creatorMiranda Oliveira, Bruno Cesar-
Autor(es): dc.creatorPeixinho, Alan Zanoni-
Autor(es): dc.creatorNagase Suzuki, Celso Tetsuo-
Autor(es): dc.creatorSaraiva Bresciani, Katia Denise-
Autor(es): dc.creatorFalcao, Alexandre Xavier-
Autor(es): dc.creatorGomes, Jancarlo Ferreira-
Data de aceite: dc.date.accessioned2021-03-11T01:12:11Z-
Data de disponibilização: dc.date.available2021-03-11T01:12:11Z-
Data de envio: dc.date.issued2019-10-04-
Data de envio: dc.date.issued2019-10-04-
Data de envio: dc.date.issued2019-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/tmi.13215-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/184529-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/184529-
Descrição: dc.descriptionWriting Department of UNICAMP-
Descrição: dc.descriptionLIDS laboratory of UNICAMP-
Descrição: dc.descriptionImmunoCamp Science and Technology-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionobjectives Laboratory diagnosis of Schistosoma mansoni eggs is routinely performed by conventional quantitative techniques through the parasitological examination of human faeces. However, the diagnostic sensitivity of this type of exam varies from low to moderate. We aimed to develop a new parasitological technique called TF-Test Quantified (TFT-Quant), for the quantitative detection of S. mansoni eggs in human faeces. methods Four study stages were performed using 43 mice infected by S. mansoni cercariae. These experiments allowed the definition of an operational protocol for TFT-Quant and a comparison of this new technique to the conventional Helm-Teste technique. results The results show a good diagnostic efficacy of TFT-Quant, which reached 100% in sensitivity and specificity, indicating an ` Almost Perfect' Kappa (k) agreement. conclusions This new technique provided a quantitative gain in the detection of S. mansoni eggs, largely free of faecal debris. The next stage of this study is the field validation of the TFT-Quant technique with the use of human faecal samples from an endemic region for mansonic schistosomiasis in Brazil (Microregion Jequitinhonha, State of Minas Gerais). In parallel to this validation, computational algorithms will be developed to allow the automated quantitative diagnosis of S. mansoni eggs.-
Formato: dc.format586-595-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-Blackwell-
Relação: dc.relationTropical Medicine & International Health-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectTFT-Quant-
Palavras-chave: dc.subjectlaboratory diagnosis-
Palavras-chave: dc.subjectSchistosoma mansoni-
Título: dc.titleTF-Test Quantified: a new technique for diagnosis of Schistosoma mansoni eggs-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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