Aging impairs osteoblast differentiation of mesenchymal stem cells grown on titanium by favoring adipogenesis

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorFlores Abuna, Rodrigo Paolo-
Autor(es): dc.creatorStringhetta-Garcia, Camila Tami-
Autor(es): dc.creatorFiori, Leonardo Pimentel-
Autor(es): dc.creatorMenegati Dornelles, Rita Cassia-
Autor(es): dc.creatorRosa, Adalberto Luiz-
Autor(es): dc.creatorBeloti, Marcio Mateus-
Data de aceite: dc.date.accessioned2021-03-11T00:59:39Z-
Data de disponibilização: dc.date.available2021-03-11T00:59:39Z-
Data de envio: dc.date.issued2018-11-26-
Data de envio: dc.date.issued2018-11-12-
Data de envio: dc.date.issued2018-11-26-
Data de envio: dc.date.issued2018-11-12-
Data de envio: dc.date.issued2016-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/1678.775720160037-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/180764-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/180764-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionProcesso FAPESP: 2012/01291-6-
Descrição: dc.descriptionCNPq: 153364/2012-2-
Descrição: dc.descriptionAging negatively affects bone/titanium implant interactions. Our hypothesis is that the unbalance between osteogenesis and adipogenesis induced by aging may be involved in this phenomenon. Objective: We investigated the osteoblast and adipocyte differentiation of mesenchymal stem cells (MSCs) from young and aged rats cultured on Ti. Material and Methods: Bone marrow MSCs derived from 1-month and 21-month rats were cultured on Ti discs under osteogenic conditions for periods of up to 21 days and osteoblast and adipocyte markers were evaluated. Results: Cell proliferation, alkaline phosphatase (ALP) activity, extracellular matrix mineralization and gene expression of RUNX2, osterix, ALP, bone sialoprotein, osteopontin, and osteocalcin were reduced in cultures of 21-month rats compared with 1-month rats grown on Ti. Gene expression of PPAR-gamma , adipocyte protein 2, and resistin and lipid accumulation were increased in cultures of 21-month rats compared with 1-month rats grown on the same conditions. Conclusions: These results indicate that the lower osteogenic potential of MSCs derived from aged rats compared with young rats goes along with the higher adipogenic potential in cultures grown on Ti surface. This unbalance between osteoblast and adipocyte differentiation should be considered in dental implant therapy to the elderly population.-
Formato: dc.format376-382-
Idioma: dc.languageen-
Publicador: dc.publisherUniv Sao Paulo Fac Odontologia Bauru-
Relação: dc.relationJournal of Applied Oral Science-
Relação: dc.relation0,645-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subjectAging-
Palavras-chave: dc.subjectOsteoblasts-
Palavras-chave: dc.subjectAdipocytes-
Palavras-chave: dc.subjectStem cells-
Palavras-chave: dc.subjectDental implants-
Palavras-chave: dc.subjectTitanium-
Título: dc.titleAging impairs osteoblast differentiation of mesenchymal stem cells grown on titanium by favoring adipogenesis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.