Zika Immunoassay Based on Surface-Enhanced Raman Scattering Nanoprobes

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Victoria (UVic)-
Autor(es): dc.creatorCamacho, Sabrina A. [UNESP]-
Autor(es): dc.creatorSobral-Filho, Regivaldo Gomes-
Autor(es): dc.creatorAoki, Pedro Henrique B. [UNESP]-
Autor(es): dc.creatorConstantino, Carlos José L. [UNESP]-
Autor(es): dc.creatorBrolo, Alexandre G.-
Data de aceite: dc.date.accessioned2022-02-21T23:36:23Z-
Data de disponibilização: dc.date.available2022-02-21T23:36:23Z-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2018-03-23-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acssensors.7b00639-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/179705-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/179705-
Descrição: dc.descriptionZika virus (ZIKV) was considered a public health emergency of international concern after the 2015 outbreak. Serological tests based on immunoassay platforms is one of the methods applied on the diagnosis of ZIKV and Dengue virus (DENV). However, the high limits of detection (LOD) and the cross-reactivity between ZIKV and DENV are still limitations in immunological tests. In order to tackle these issues, we have designed an immune-specific assay based on surface-enhanced Raman scattering (SERS) nanoprobes. Gold shell-isolated nanoparticles (Au-SHINs) were synthesized with 100 nm Au nanoparticles and 4 nm silica shell thickness coated with Nile Blue (Raman reporter). Then, the SERS nanoprobes were wrapped in a final silica shell and functionalized with monoclonal anti-ZIKV NS1 antibodies. Concentrations of ZIKV NS1 down to 10 ng/mL were probed free of cross-reactivity with DENV NS1 antigens.-
Descrição: dc.descriptionSchool of Technology and Applied Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Chemistry Center for Advanced Materials and Related Technologies (CAMTEC) University of Victoria (UVic)-
Descrição: dc.descriptionSchool of Sciences Humanities and Languages São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Technology and Applied Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Sciences Humanities and Languages São Paulo State University (UNESP)-
Formato: dc.format587-594-
Idioma: dc.languageen-
Relação: dc.relationACS Sensors-
Relação: dc.relation1,895-
Direitos: dc.rightsAcesso restrito-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcross-reactivity-
Palavras-chave: dc.subjectgold shell-isolated nanoparticles-
Palavras-chave: dc.subjectRaman reporter-
Palavras-chave: dc.subjectSERS nanoprobes-
Palavras-chave: dc.subjectZika detection-
Título: dc.titleZika Immunoassay Based on Surface-Enhanced Raman Scattering Nanoprobes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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