Regulatory and junctional proteins of the blood-testis barrier in human Sertoli cells are modified by monobutyl phthalate (MBP) and bisphenol A (BPA) exposure

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorde Freitas, André Teves Aquino Gonçalves-
Autor(es): dc.creatorRibeiro, Mariana Antunes-
Autor(es): dc.creatorPinho, Cristiane Figueiredo-
Autor(es): dc.creatorPeixoto, André Rebelo-
Autor(es): dc.creatorDomeniconi, Raquel Fantin-
Autor(es): dc.creatorScarano, Wellerson R.-
Data de aceite: dc.date.accessioned2021-03-11T00:52:35Z-
Data de disponibilização: dc.date.available2021-03-11T00:52:35Z-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2016-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.tiv.2016.02.017-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/177908-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/177908-
Descrição: dc.descriptionThe blood-testis barrier (BTB) is responsible for providing a protected environment and coordinating the spermatogenesis. Endocrine disruptors (EDs) might lead to infertility, interfering in the BTB structure and modulation. This study aimed to correlate the actions of two EDs, monobutyl phthalate (MBP) and bisphenol A (BPA) in different periods of exposure, in a low toxicity dose to the human Sertoli cells (HSeC) and its effects on the proteins of the BTB and regulatory proteins involved in its modulation. HSeC cells were exposed to MBP (10 μM) and BPA (20 μM) for 6 and 48 h. Western Blot assay indicated that MBP was able to reduce the expression of occludin, ZO-1, N-cadherin and Androgen Receptor (AR), while BPA leads to a reduction of occludin, ZO-1, β-catenin and AR. TGF-β2 and F-actin were not modified. Phalloidin and Hematoxylin and Eosin assay revealed phenotically disruption in Sertoli cells adhesion, without changes in F-actin expression or localization. Our data suggested both EDs present potential for disrupting the structure and maintenance of the human BTB by AR dependent pathway.-
Formato: dc.format1-7-
Idioma: dc.languageen-
Relação: dc.relationToxicology in Vitro-
Relação: dc.relation0,931-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subject(HSeC) Human Sertoli cells-
Palavras-chave: dc.subjectBisphenol A-
Palavras-chave: dc.subjectBlood-testis barrier-
Palavras-chave: dc.subjectEndocrine disruptors-
Palavras-chave: dc.subjectMale reproductive function-
Palavras-chave: dc.subjectMonobutyl phthalate-
Título: dc.titleRegulatory and junctional proteins of the blood-testis barrier in human Sertoli cells are modified by monobutyl phthalate (MBP) and bisphenol A (BPA) exposure-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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