Determination of Cephalosporins by UHPLC-DAD Using Molecularly Imprinted Polymers

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBaeza-Fonte, Alen N-
Autor(es): dc.creatorGarcés-Lobo, Idenia-
Autor(es): dc.creatorLuaces-Alberto, Markel D-
Autor(es): dc.creatorGonçalves, Luís Moreira-
Autor(es): dc.creatorSotomayor, Maria D P T-
Autor(es): dc.creatorValdés-González, Arístides C-
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Descrição: dc.descriptionMolecularly imprinted polymers (MIPs) were synthesized for the determination of cephalosporins (cephazolin, ceftazidime, cefotaxime, ceftriaxone, cefepime and cephalexin) by ultra-high performance liquid chromatography with diode-array detection (UHPLC-DAD). After optimization, MIPs were synthesized using cephazolin as the template, methacrylic acid as the functional monomer, triethylenglycol dimethacrylate as the crosslinker, acetonitrile/dimethylsulfoxide as porogens and benzoyl peroxide as the radical initiator. Not only this is a novel route of MIP synthesis for cephalosporins, but also this choice of analytes is unique. Chromatographic separation was performed in a C 8 column using a binary gradient with trifluoroacetic acid 0.1% in water and acetonitrile. Linearity was assessed up to 100 μg mL -1 and linear correlation coefficients (r) were all higher than 0.99, limits of detection were within the range of 3-12 ng mL -1, and recoveries from 86 to 102% were obtained for concentrations between 0.05 and 1.0 μg mL -1.-
Formato: dc.format187-193-
Idioma: dc.languageen-
Relação: dc.relationJournal of Chromatographic Science-
Relação: dc.relation0,360-
Direitos: dc.rightsopenAccess-
Título: dc.titleDetermination of Cephalosporins by UHPLC-DAD Using Molecularly Imprinted Polymers-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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