Sperm membrane integrity and stability after selection of cryopreserved ovine semen on colloidal solutions

Registro completo de metadados
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBergstein-Galan, T. G.-
Autor(es): dc.creatorBicudo, L. C.-
Autor(es): dc.creatorRodello, L.-
Autor(es): dc.creatorWeiss, R. R.-
Autor(es): dc.creatorBicudo, S. D.-
Data de aceite: dc.date.accessioned2021-03-11T00:45:30Z-
Data de disponibilização: dc.date.available2021-03-11T00:45:30Z-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2018-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/and.12867-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/174973-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/174973-
Descrição: dc.descriptionThe aim of this study was to evaluate the effect of four methods of sperm selection, on the integrity and stability of the plasma membrane, integrity of the acrosomal membrane and spermatic morphology in frozen/thawed ovine semen. Two types of colloidal silica: colloidal silica–silane and colloidal silica–polyvinylpyrrolidone (PVP), and two aliquots: 1 and 4 ml, were used for sperm selection. Probes FITC-PSA and PI were used to measure the integrity of the plasma and acrosomal membranes. Plasma membrane stability was measured, using fluorescent probes M540 and YOPRO1. Effective reduction in the incidence of spermatozoa with acrosomal pathologies was only achieved using 1 ml colloidal silica–silane. All methods were efficient in select viable and unreacted spermatozoa. Only methods using 1 ml of silica were efficient in decrease spermatozoa stained by PI (death). Methods using silica colloidal–silane were more efficient to decrease apoptotic cells after selection when compared to silica colloidal–PVP. In conclusion, sperm selection in colloidal silica–silane and colloidal silica–PVP improved sperm quality when compared to the controls. The method using 1 ml of colloidal silica–silane is the preferred method because its effectiveness and lower cost.-
Idioma: dc.languageen-
Relação: dc.relationAndrologia-
Relação: dc.relation0,623-
Relação: dc.relation0,623-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectcolloidal silica-
Palavras-chave: dc.subjectsperm membranes-
Palavras-chave: dc.subjectsperm selection-
Título: dc.titleSperm membrane integrity and stability after selection of cryopreserved ovine semen on colloidal solutions-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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