Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPizauro, Lucas J.L.-
Autor(es): dc.creatorde Almeida, Camila C.-
Autor(es): dc.creatorSoltes, Glenn A.-
Autor(es): dc.creatorSlavic, Durda-
Autor(es): dc.creatorRossi-Junior, Oswaldo D.-
Autor(es): dc.creatorde Ávila, Fernando. A.-
Autor(es): dc.creatorZafalon, Luiz. F.-
Autor(es): dc.creatorMacInnes, Janet I.-
Data de aceite: dc.date.accessioned2021-03-11T00:44:13Z-
Data de disponibilização: dc.date.available2021-03-11T00:44:13Z-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2017-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.vetmic.2017.03.036-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/174461-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/174461-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionIncorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1.99) using MALDI-TOF MS. However, as determined by cytochrome d ubiquinol oxidase subunit II (cydB) qPCR and by 16S RNA and cydB gene sequencing, 10 S. agnetis strains were wrongly identified as S. hyicus by MALDI-TOF MS. In addition, 9 isolates identified by MALDI-TOF only to the genus level (score values between 1.70 and 1.99) could be identified to species by cydB qPCR. Our findings suggest that MALDI-TOF MS is a reliable method for rapid identification of S. chromogenes and S. epidermidis (species of interest both in human and veterinary medicine) and may be able to correctly identify other Staphylococcus spp. However, at present not all Staphylococcus spp. found in buffalo milk can be accurately identified by MALDI-TOF MS and for these organisms, the cydB qPCR developed in the current study may provide a reliable alternative method for rapid identification of CoNS species.-
Formato: dc.format8-14-
Idioma: dc.languageen-
Relação: dc.relationVeterinary Microbiology-
Relação: dc.relation1,175-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subjectcydB qPCR-
Palavras-chave: dc.subjectMALDI-TOF MS-
Palavras-chave: dc.subjectMilk-
Palavras-chave: dc.subjectStaphylococcus agnetis-
Palavras-chave: dc.subjectStaphylococcus hyicus-
Título: dc.titleSpecies level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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