Efeito do fator de crescimento epidermal (EGF) na maturação in vitro de oócitos caninos

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPereira, Leda Maria Costa-
Autor(es): dc.creatorBersano, Paulo Ricardo de Oliveira-
Autor(es): dc.creatorLopes, Maria Denise-
Data de aceite: dc.date.accessioned2021-03-11T00:37:21Z-
Data de disponibilização: dc.date.available2021-03-11T00:37:21Z-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2014-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.11606/issn.1678-4456.v51i2p158-165-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/171682-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/171682-
Descrição: dc.descriptionThis work aimed to evaluate the influence of EGF on canine oocyte in vitro maturation (IVM). We carried out the transport of the ovaries in sodium chloride 0.9% solution, which were cut into phosphate-buffered saline (PBS) and the cumulus oocyte complexes (COCs) selected in tissue culture medium (TCM), supplemented with 199 HEPES. A total of 405 grade 1 oocytes were collected from bitches in anestrus and diestrus. Oocytes from the two phases of estrous cycle were subjected to two treatments: medium with addition of 10 ηg/mL epidermal growth factor (EGF) (T) and medium without supplementation (C). After 72 h of maturation, COCs were denuded, fixed and stained with Hoechst 33342 to assess nuclear maturation. The oocytes obtained from the ovaries of the diestrus phase of the T group demonstrated higher percentage (18.8%) of oocytes at metaphase II stage (M-II) than C group (1.3%) (p < 0.01). The oocytes from anestrus phase demonstrated difference (p < 0.05) between the groups, observing smaller proportion (4.1%) of oocytes at the stage of germinal vesicle (GV) in group T compared to group C (16.1%). Comparing the different reproductive status in media supplemented with EGF difference (p < 0.05) was observed only in oocytes diestrus phase relative to the stage of M-II. Thus, supplementation on maturation medium at a concentration of 10 ηg/mL of EGF in this study had positive influence only on IVM of canine oocytes obtained from diestrus phase, increasing the levels of M-II in this species.-
Formato: dc.format158-165-
Idioma: dc.languagept_BR-
Relação: dc.relationBrazilian Journal of Veterinary Research and Animal Science-
Relação: dc.relation0,225-
Relação: dc.relation0,225-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectAnestrus-
Palavras-chave: dc.subjectBitch-
Palavras-chave: dc.subjectDiestrus-
Palavras-chave: dc.subjectEGF-
Palavras-chave: dc.subjectIn vitro maturation-
Título: dc.titleEfeito do fator de crescimento epidermal (EGF) na maturação in vitro de oócitos caninos-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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