Host-pathogen interactions in bovine mammary epithelial cells and HeLa cells by Staphylococcus aureus isolated from subclinical bovine mastitis

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorCastilho, Ivana G.-
Autor(es): dc.creatorAlves Dantas, Stefani Thais-
Autor(es): dc.creatorLangoni, Helio-
Autor(es): dc.creatorAraujo, Joao P.-
Autor(es): dc.creatorFernandes, Ary-
Autor(es): dc.creatorAlvarenga, Fernanda C. L.-
Autor(es): dc.creatorMaia, Leandro-
Autor(es): dc.creatorCagnini, Didier Q.-
Autor(es): dc.creatorRall, Vera L. M.-
Data de aceite: dc.date.accessioned2021-03-10T23:55:00Z-
Data de disponibilização: dc.date.available2021-03-10T23:55:00Z-
Data de envio: dc.date.issued2018-11-26-
Data de envio: dc.date.issued2018-11-26-
Data de envio: dc.date.issued2017-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3168/jds.2017-12700-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/163007-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/163007-
Descrição: dc.descriptionStaphylococcus aureus is a common pathogen that causes subclinical bovine mastitis due to several virulence factors. In this study, we analyzed S. aureus isolates collected from the milk of cows with subclinical mastitis that had 8 possible combinations of bap, icaA, and icaD genes, to determine their capacity to produce biofilm on biotic (bovine primary mammary epithelial cells and HeLa cells) and abiotic (polystyrene microplates) surfaces, and their ability to adhere to and invade these cells. We also characterized isolates for microbial surface components recognizing adhesive matrix molecules (MSCRAMM) and agr genes, and for their susceptibility to cefquinome sulfate in the presence of biofilm. All isolates adhered to and invaded both cell types, but invasion indexes were higher in bovine primary mammary epithelial cells. Using tryptic soy broth + 1% glucose on abiotic surfaces, 5 out of 8 isolates were biofilm producers, but only the bap(+)icail(+)icaD(+) isolate was positive in Dulbecco's Modified Eagle's medium. The production of biofilm on biotic surfaces occurred only with this isolate and only on HeLa cells, because the invasion index for bovine primary mammary epithelial cells was too high, making it impossible to use these cells in this assay. Of the 5 biofilm producers in tryptic soy broth + 1% glucose, 4 presented with the bapl /fnbA / clfA/ clfB / enol / fibl / ebpS combination, and all were protected from cefquinome sulfate. We found no predominance of any agr group. The high invasive potential of S. aureus made it impossible to observe biofilm in bovine primary mammary epithelial cells, and we concluded that cells with lower invasion rates, such as HeLa cells, were more appropriate for this assay.-
Formato: dc.format6414-6421-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationJournal Of Dairy Science-
Relação: dc.relation1,350-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectmicrobial surface components recognizing adhesive matrix molecules (MSCRAMM)-
Palavras-chave: dc.subjectagr allotype-
Palavras-chave: dc.subjectmastitis-
Palavras-chave: dc.subjectbiofilm-
Palavras-chave: dc.subjectresistance-
Título: dc.titleHost-pathogen interactions in bovine mammary epithelial cells and HeLa cells by Staphylococcus aureus isolated from subclinical bovine mastitis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.