Metabolic Effects of Cobalt Ferrite Nanoparticles on Cervical Carcinoma Cells and Nontumorigenic Keratinocytes

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBortolozo Oliveira, Ana Beatriz-
Autor(es): dc.creatorMoraes, Fabio Rogerio de-
Autor(es): dc.creatorCandido, Natalia Maria-
Autor(es): dc.creatorSampaio, Isabella-
Autor(es): dc.creatorPaula, Alex Silva-
Autor(es): dc.creatorVasconcellos, Adrian de-
Autor(es): dc.creatorSilva, Thais Cerqueira-
Autor(es): dc.creatorMiller, Alex Henrique-
Autor(es): dc.creatorRahal, Paula-
Autor(es): dc.creatorNery, Jose Geraldo-
Autor(es): dc.creatorCalmon, Martha Freitas-
Data de aceite: dc.date.accessioned2021-03-10T23:52:39Z-
Data de disponibilização: dc.date.available2021-03-10T23:52:39Z-
Data de envio: dc.date.issued2018-11-26-
Data de envio: dc.date.issued2018-11-26-
Data de envio: dc.date.issued2016-12-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acs.jproteome.6b00411-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/162226-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/162226-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionProcesso FAPESP: 2012/22809-3-
Descrição: dc.descriptionProcesso FAPESP: 2009/53989-4-
Descrição: dc.descriptionThe cytotoxic response, cellular uptake, and metabolomic profile of HeLa and HaCaT cell lines treated with cobalt ferrite nanoparticles (CoFe2O4 NPs) were investigated in this study. Cell viability assays showed low cytotoxicity caused by the uptake of the nanoparticles at 2 mg/mL. However, metabolomics revealed that these nanoparticles impacted cell metabolism even when tested at a concentration that presented low cytotoxicity according to the cell viability assay. The two cell lines shared stress-related metabolic changes such as increase in alanine and creatine levels. A reduced level of fumarate was also observed in HeLa cells after treatment with the nanoparticles, and this alteration can inhibit tumorigenesis. Fumarate is considered to be an oncometabolite that can inhibit prolyl hydroxylase, and this inhibition stabilizes HIF1 alpha, one of the master regulators of tumorigenesis that promotes tumor growth and development. In summary, this study showed that nanoparticle-treated HeLa cells demonstrated decreased concentrations of metabolites associated with cell proliferation and tumor growth. The results clearly indicated that treatment with these nanoparticles might cause a perturbation in cellular metabolism.-
Formato: dc.format4337-4348-
Idioma: dc.languageen-
Publicador: dc.publisherAmer Chemical Soc-
Relação: dc.relationJournal Of Proteome Research-
Relação: dc.relation1,818-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectcobalt ferrite nanoparticles-
Palavras-chave: dc.subjectcell lines-
Palavras-chave: dc.subjectcytotoxicity-
Palavras-chave: dc.subjectcell uptake-
Palavras-chave: dc.subjectmetabolomics-
Título: dc.titleMetabolic Effects of Cobalt Ferrite Nanoparticles on Cervical Carcinoma Cells and Nontumorigenic Keratinocytes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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