Sunflower HaGPAT9-1 is the predominant GPAT during seed development

Registro completo de metadados
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPaya-Milans, Miriam-
Autor(es): dc.creatorAntonio Aznar-Moreno, Jose-
Autor(es): dc.creatorBalbuena, Tiago S.-
Autor(es): dc.creatorHaslam, Richard P.-
Autor(es): dc.creatorGidda, Satinder K.-
Autor(es): dc.creatorPerez-Hormaeche, Javier-
Autor(es): dc.creatorMullen, Robert T.-
Autor(es): dc.creatorThelen, Jay J.-
Autor(es): dc.creatorNapier, Johnathan A.-
Autor(es): dc.creatorSalas, Joaquin J.-
Autor(es): dc.creatorGarces, Rafael-
Autor(es): dc.creatorMartinez-Force, Enrique-
Autor(es): dc.creatorVenegas-Caleron, Monica-
Data de aceite:
Data de disponibilização:
Data de envio:
Data de envio:
Data de envio:
Fonte completa do material: dc.identifier
Fonte completa do material: dc.identifier
Fonte: dc.identifier.uri
Descrição: dc.descriptionFEDER [JAE-CSIC]-
Descrição: dc.descriptionBiotechnology and Biological Sciences Research Council-
Descrição: dc.descriptionFEDER [JAE-CSIC]: AGL2014-53537-R-
Descrição: dc.descriptionBiotechnology and Biological Sciences Research Council: BBS/E/C/00005207-
Descrição: dc.descriptionIn oil crops, triacylglycerol biosynthesis is an important metabolic pathway in which glycerol-3-phosphate acyltransferase (GPAT) performs the first acylation step. Mass spectrometry analysis of developing sunflower (Helianthus annuus) seed membrane fractions identified an abundant GPAT, HaGPAT9 isoform 1, with a N-terminal peptide that possessed two phosphorylated residues with possible regulatory function. HaGPAT9-1 belongs to a broad eukaryotic GPAT family, similar to mammalian GPAT3, and it represents one of the two sunflower GPAT9 isoforms, sharing 90% identity with HaGPAT9-2. Both sunflower genes are expressed during seed development and in vegetative tissues, with HaGPAT9-1 transcripts accumulating at relatively higher levels than those for HaGPAT9-2. Green fluorescent protein tagging of HaGPAT9-1 confirmed its subcellular accumulation in the endoplasmic reticulum. Despite their overall sequence similarities, the two sunflower isoforms displayed significant differences in their enzymatic activities. For instance, HaGPAT9-1 possesses in vivo GPAT activity that rescues the lethal phenotype of the cmy228 yeast strain, while in vitro assays revealed a preference of HaGPAT9-1 for palmitoyl-, oleoyl- and linoleoyl-CoAs of one order of magnitude, with the highest increase in yield for oleoyl- and linoleoyl-CoAs. By contrast, no enzymatic activity could be detected for HaGPAT9-2, even though its over-expression modified the TAG profile of yeast. (C) 2016 Elsevier Ireland Ltd. All rights reserved.-
Formato: dc.format42-52-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationPlant Science-
Relação: dc.relation1,667-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subjectEndoplasmic reticulum-
Palavras-chave: dc.subjectGlycerol-3-phosphate acyltransferase-
Palavras-chave: dc.subjectHelianthus annuus-
Palavras-chave: dc.subjectMass spectrometry-
Palavras-chave: dc.subjectTriacylglycerol-
Palavras-chave: dc.subjectYeast-
Título: dc.titleSunflower HaGPAT9-1 is the predominant GPAT during seed development-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.