Viability of ovine spermatozoa collected from epididymides stored at 18 degrees-25 degrees C for 48 hours post mortem

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBergstein-Galan, T. G.-
Autor(es): dc.creatorWeiss, R. R.-
Autor(es): dc.creatorBarbosa, T. S. R.-
Autor(es): dc.creatorKozicki, L. E.-
Autor(es): dc.creatorBicudo, S. D.-
Data de aceite: dc.date.accessioned2021-03-10T23:47:44Z-
Data de disponibilização: dc.date.available2021-03-10T23:47:44Z-
Data de envio: dc.date.issued2018-11-26-
Data de envio: dc.date.issued2018-11-26-
Data de envio: dc.date.issued2018-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/1678-4162-10058-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/160474-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/160474-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionThe objectives of this study were to verify the time during which viable ovine spermatozoa could be recovered from the cauda epididymis kept at ambient temperature (18-25 degrees C). Sperm collected in an artificial vagina (AV) were used as control. Spermatozoa samples were collected with an AV and from epididymis at 0 (G0), 6 (G6), 12 (G12), 24 (G24), and 48 (G48) hours post mortem. Total motility (TM), progressive motility (PM), hypo-osmotic membrane integrity test (HOST) and morphological changes were assessed. TM decreased (P<0.05) from 24 hours post mortem (70.0 +/- 1.9%) compared to AV (86.4 +/- 1.0%). PM decreased (P<0.05) from 12 hours after death (31.3 +/- 4.0%) compared to AV group (73.2 +/- 1.4%). The percentage of viable cells in HOST decreased (P<0.05) in the G48 (60.0 +/- 8.9%). Spermatozoa recovery was lower (P<0.05) 48 hours after death (2064.2 +/- 230.7 x 10(6) spermatozoa) compared to G0(2623.6 +/- 288.4 x 10(6) spermatozoa). In conclusion, under the conditions of this study, it would be possible to use epididymal spermatozoa recovered up to 24 hours after death for artificial insemination or in vitro fertilization; however, fertility trials are necessary to prove this hypothesis.-
Formato: dc.format1023-1028-
Idioma: dc.languageen-
Publicador: dc.publisherArquivo Brasileiro Medicina Veterinaria Zootecnia-
Relação: dc.relationArquivo Brasileiro De Medicina Veterinaria E Zootecnia-
Relação: dc.relation0,248-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subjectram-
Palavras-chave: dc.subjectepididymis-
Palavras-chave: dc.subjectroom temperature-
Título: dc.titleViability of ovine spermatozoa collected from epididymides stored at 18 degrees-25 degrees C for 48 hours post mortem-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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