Electrochemical immunosensor for ethinylestradiol using diazonium salt grafting onto silver nanoparticles-silica-graphene oxide hybrids

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorCincotto, Fernando H.-
Autor(es): dc.creatorMartinez-Garcia, Gonzalo-
Autor(es): dc.creatorYanez-Sedeno, Paloma-
Autor(es): dc.creatorCanevari, Thiago. C.-
Autor(es): dc.creatorMachado, S. A. S.-
Autor(es): dc.creatorPingarron, Jose M.-
Data de aceite: dc.date.accessioned2021-03-10T23:43:16Z-
Data de disponibilização: dc.date.available2021-03-10T23:43:16Z-
Data de envio: dc.date.issued2018-11-26-
Data de envio: dc.date.issued2018-11-26-
Data de envio: dc.date.issued2016-01-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.talanta.2015.09.061-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/158619-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/158619-
Descrição: dc.descriptionSpanish Ministerio de Economia y Competitividad-
Descrição: dc.descriptionComunidad de Madrid-
Descrição: dc.descriptionSpanish Ministerio de Economia y Competitividad: CTQ2012-35041-
Descrição: dc.descriptionComunidad de Madrid: S2013/MT-3029-
Descrição: dc.descriptionThis work describes the preparation of an electrochemical immunosensor for ethinylestradiol (EE2) based on grafting of diazonium salt of 4-aminobenzoic acid onto a glassy carbon electrode modified with silver nanoparticles/SiO2/graphene oxide hybrid followed by covalent binding of anti-ethinylestradiol (anti-EE2) to activated carboxyl groups. A competitive immunoassay was developed for the determination of the hormone using peroxidase-labeled ethinylestradiol (HRP-EE2) and measurement of the amperometric response at -200 mV in the presence of hydroquinone (HQ) as redox mediator. The calibration curve for EE2 exhibited a linear range between 0.1 and 50 ng/mL (r(2)=0.996), with a detection limit of 65 pg/mL. Interference studies with other hormones related with EE2 revealed the practical specificity of the developed method for the analyte. A good reproducibility, with RSD=4.5% (n=10) was also observed. The operating stability of a single bioelectrode modified with anti-EE2 was maintained at least for 15 days when it was stored at 4 degrees C under humid conditions between measurements. The developed immunosensor was applied to the analysis of spiked urine with good results. (C) 2015 Elsevier B.V. All rights reserved.-
Formato: dc.format328-334-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationTalanta-
Relação: dc.relation1,186-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subjectEthinylestradiol-
Palavras-chave: dc.subjectEE2-
Palavras-chave: dc.subjectElectrochemical immunosensor-
Palavras-chave: dc.subjectSilver nanoparticles-
Palavras-chave: dc.subjectGraphene oxide-
Palavras-chave: dc.subjectUrine-
Título: dc.titleElectrochemical immunosensor for ethinylestradiol using diazonium salt grafting onto silver nanoparticles-silica-graphene oxide hybrids-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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