Bioanalytical methods for the metalloproteomics study of bovine longissimus thoracis muscle tissue with different grades of meat tenderness in the Nellore breed (Bos indicus)

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBaldassini, Welder Angelo-
Autor(es): dc.creatorBraga, Camila Pereira-
Autor(es): dc.creatorLoyola Chardulo, Luis Artur-
Autor(es): dc.creatorIi Vasconcelos Silva, Josineudson Augusto-
Autor(es): dc.creatorMalheiros, Jessica Moraes-
Autor(es): dc.creatorAlbuquerque, Lucia Galvao de-
Autor(es): dc.creatorFernandes, Talita Tanaka-
Autor(es): dc.creatorPadilha, Pedro de Magalhaes-
Data de aceite: dc.date.accessioned2021-03-10T22:06:09Z-
Data de disponibilização: dc.date.available2021-03-10T22:06:09Z-
Data de envio: dc.date.issued2015-10-21-
Data de envio: dc.date.issued2015-10-21-
Data de envio: dc.date.issued2015-02-15-
Fonte completa do material: dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0308814614011790-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/128440-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/128440-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionInstitute of Biosciences Foundation (FUNDIBIO)-
Descrição: dc.descriptionProcesso FAPESP: 2009/16118-5-
Descrição: dc.descriptionProcesso FAPESP: 2010/51332-5-
Descrição: dc.descriptionProcesso FAPESP: 2011/14850-0-
Descrição: dc.descriptionThe work describes a metalloproteomics study of bovine muscle tissue with different grades of meat tenderness from animals of the Nellore breed (Bos indicus) based on protein separation by two-dimensional gel electrophoresis, the identification of calcium ions in protein spots by X-ray fluorescence (SR-XRF) and the characterisation of proteins by electrospray ionisation mass spectrometry. Forty (40) specimens were selected and divided into two experimental groups: animals with tough meat (TO) and animals with tender meat (TE). A third group (P) of Piedmontese breed animals (Bos taurus) was included to serve as a comparative model for the level of meat tenderness. The procedures were efficient and preserved the metal protein structure, enabling calcium detection in protein spots by SR-XRF at a given molecular weight range of 14-97 kDa. Two proteins (pyruvate kinase and albumin) were inferred to be related to the phenotypical differences in animals from the different groups. (C) 2014 Elsevier Ltd. All rights reserved.-
Formato: dc.format65-72-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier B.V.-
Relação: dc.relationFood Chemistry-
Relação: dc.relation4.946-
Relação: dc.relation1,793-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subject2D-PAGE-
Palavras-chave: dc.subjectBeef cattle-
Palavras-chave: dc.subjectCalcium-
Palavras-chave: dc.subjectMass spectrometry-
Palavras-chave: dc.subjectZebu genotype-
Título: dc.titleBioanalytical methods for the metalloproteomics study of bovine longissimus thoracis muscle tissue with different grades of meat tenderness in the Nellore breed (Bos indicus)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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