Comparison of lipase production on crambe oil and meal by Fusarium sp (Gibberella fujikuroi complex)

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorOliveira, Bruno H.-
Autor(es): dc.creatorCoradi, Gilberto V.-
Autor(es): dc.creatorAttili-Angelis, Derlene-
Autor(es): dc.creatorScauri, Carolina-
Autor(es): dc.creatorLuques, Ana Helena P. G.-
Autor(es): dc.creatorBarbosa, Aneli M.-
Autor(es): dc.creatorDekker, Robert F. H.-
Autor(es): dc.creatorOliva Neto, Pedro de-
Autor(es): dc.creatorLima, Valeria M. G.-
Data de aceite: dc.date.accessioned2021-03-10T21:22:04Z-
Data de disponibilização: dc.date.available2021-03-10T21:22:04Z-
Data de envio: dc.date.issued2014-12-03-
Data de envio: dc.date.issued2014-12-03-
Data de envio: dc.date.issued2013-12-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/ejlt.201300087-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/113337-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/113337-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionProcesso FAPESP: 10/07998-9-
Descrição: dc.descriptionThis study provides a comparison of fermentation conditions for the production lipases by Fusarium sp. (Gibberella fujikuroi complex) isolate FCLA-MA-41 in submerged (SmF) and solid-state fermentation (SSF) using agro-industrial residues. To assess SmF, a univariate study of seven carbon sources (crambe, corn, linseed, olive, palm (dende), soybean oils and chicken fat) and four nitrogen sources (ammonium sulfate, sodium nitrate, urea, yeast extract) was performed. The relationship among the concentrations of carbon and nitrogen sources, Triton X-100 and yeast extract was studied using a Central Composite Design (CCD). To assess SSF, different residues (sugarcane bagasse) and oil-seed meals (castorbean, corn, crambe, soybean) with additional supplements added (minimum salts, seed oils) were examined. Based on the CCD matrix, a medium containing crambe oil (17.5mL/L), Triton X-100 (5g/L), ammonium sulfate (5g/L) and yeast extract (1g/L) was proposed, resulting in a lipase titer of 3.0 +/- 0.25U/mL. The cost/production relationship was 7.73% less expensive than SmF with corn oil. SSF produced a maximum lipase titer of 5.0 +/- 0.25U/gds on crambe meal moistened with phosphate buffer. The best cost/enzyme activity estimate was for SSF with crambe meal as substrate in only distilled water (87.27% less expensive than SmF).-
Formato: dc.format1413-1425-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-Blackwell-
Relação: dc.relationEuropean Journal of Lipid Science and Technology-
Relação: dc.relation2.200-
Relação: dc.relation0,776-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectCentral composite design-
Palavras-chave: dc.subjectCorn oil-
Palavras-chave: dc.subjectChicken fat-
Palavras-chave: dc.subjectAmmonium sulfate-
Palavras-chave: dc.subjectTriton X-100-
Título: dc.titleComparison of lipase production on crambe oil and meal by Fusarium sp (Gibberella fujikuroi complex)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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