Crystallization and preliminary X-ray crystallographic analysis of importin-alpha from Neurospora crassa

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBernardes, Natalia E.-
Autor(es): dc.creatorTakeda, Agnes A. S.-
Autor(es): dc.creatorFreitas, Fernanda Zanolli-
Autor(es): dc.creatorBertolini, Maria Celia-
Autor(es): dc.creatorFontes, Marcos R. M.-
Data de aceite: dc.date.accessioned2021-03-10T21:19:59Z-
Data de disponibilização: dc.date.available2021-03-10T21:19:59Z-
Data de envio: dc.date.issued2014-12-03-
Data de envio: dc.date.issued2014-12-03-
Data de envio: dc.date.issued2014-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1107/S2053230X14005068-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/112614-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/112614-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionImportin-alpha recognizes cargo proteins that contain classical nuclear localization sequences (NLS) and, in complex with importin-beta, is able to translocate nuclear proteins through the nuclear pore complex. The filamentous fungus Neurospora crassa is a well studied organism that has been widely used as a model organism for fundamental aspects of eukaryotic biology, and is important for understanding the specific mechanisms of protein transport to the cell nucleus. In this work, the crystallization and preliminary X-ray diffraction analysis of importin-alpha from N. crassa (IMP alpha-Nc) complexed with a classical NLS peptide (SV40 NLS) are reported. IMP alpha-Nc-SV40 NLS crystals diffracted X-rays to 2.0 angstrom resolution and the structure was solved by molecular-replacement techniques, leading to a monomeric structure. The observation of the electron-density map indicated the presence of SV40 NLSs interacting at both the minor and major NLS-binding sites of the protein.-
Formato: dc.format501-504-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-Blackwell-
Relação: dc.relationActa Crystallographica Section F: Structural Biology Communications-
Direitos: dc.rightsclosedAccess-
Título: dc.titleCrystallization and preliminary X-ray crystallographic analysis of importin-alpha from Neurospora crassa-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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