MOLECULAR DETECTION OF PHYTOPLASMA ASSOCIATED WITH GREEN PÉTALO IN FRESA (Fragaria x ananassa Duchense) IN ZAMORA, MICHOACÁN (Atena Editora)

Abstract

In millet plants (Fragaria x ananassa Duch.), which exhibited symptoms of early reddening in the nursery and cultivated fields, which were collected from fields in the Valle de Zamora, Michoacán, Mexico, the presence of phytoplasmas was detected using the reaction technique anidated polymerase chain (PCR). In a first reaction with the pair of P1/P7 strainers and a second amplification (anidated PCR), of the P1/Ptint strainers, where it was possible to amplify a product of 800-850 bp. In addition, high fluorescence signals of prokaryotic bodies were observed using the DAPI staining technique. Plants with virescent flowers and plants with fruits that exhibit symptoms of non-infectious phyllody were also subjected to PCR analysis, with negative results. The samples collected were submitted to PCR with the use of specific strainers fSTOL/rSTOL, for the detection of the Stolbur phytoplasma group in a mill where the Australian phytoplasma diseases (AUSGY), lethal yellowing (StrawLY) and belonging diseases are grouped al group 16Sr XIII, virescence of the Mexican vinca (MPV) group16SrXIII-A, green petal of the fresa (SGP) group16SrXIII-B. Prokaryotic DNA was not detected with the use of the PCR technique and specific strainers fSTOL/rSTOL and DAPI staining, therefore, the presence of phytoplasmas grouped in the Stolbur phytoplasma group in samples collected in vivarium and milling fields is ruled out.