MOTHER TINCTURE OF DIFFERENT GENOTYPES OF SCHINUS TEREBINTHIFOLIA RADDI: NEW INSIGHTS FOR BIOTECHNOLOGICAL INNOVATION IN THE OPTIMIZATION OF EXTRACTION METHOD AND PHYTOCHEMICAL ANALYSIS (Atena Editora)

Abstract

-Traditional systems of medicine have become a topic of global importance. For that matter, the species Schinus terebinthifolia Raddi (aroeira vermelha or Brazilian Peppertree), constitutes an important group between medicinal and aromatic species with economic value, due to its application in the food, cosmetic and pharmaceutical industry. This study reports that new insights for biotechnological innovation in the optimization of extraction method of three genotypes (male, female and hermaphrodite) of Schinus terebinthifolia species have profound effect on the isolation of active chemical principles and impact on quality. Physico-chemical analyzes of mother tincture from leaves and fruits presented default values for determination of dry residue (1.41% ± 0.13), alcohol content (67% ± 0.78), pH (5.6 ± 0.78) and density (0.898g.mL-1 ± 0.98) of mother tinctures. The chromatographic system - thin layer chromatography - adopted in sample screening leaves and fruits, allowed the detection of chromatographic zones of major metabolic classes for phenolic groups, flavonoid type, mainly for mother tincture’s leaves. In the identification of chemical constituents of mother tincture of selected genotypes in the thin layer chromatography, was used high-performance liquid chromatography. The chromatogram for mother tincture of leaves detected metabolic groups, suggested the presence of hydrophilic compounds, as catechins and glycosylated flavonoids and for mother tincture of fruits, metabolic groups suggested the presence saponins and cardiotonic heterosides, of pyrogallic tannins (hydrolysable tannins), galotannins and tannins of ellagic acid. The best of our knowledge, this is the first report of optimization of extraction method (by extraction with “ecocertified”- AD1 adjuvant), and phytochemical analysis of different genotypes of Schinus terebinthifolia’s mother tincture as a rich source of its components that may offer interesting potential applications in the food, cosmetic, biotechnology and/or pharmaceutical industries.