ECOLOGICAL STUDY OF THE STOMATIC DENSITY OF FOREST SPECIES (Atena Editora)

Abstract

The Amazon concentrates the greatest diversity of forest species on the planet, among these species are the Adenhantera pavonina L. and the Hevea brasiliensis Muell Arg. Considering the scarcity of studies relating stomata with the physiology of these species in the literature, the objective of this work was to analyze the stomatal density and its relationship with the physiology of the species, as well as identify the types of stomata present in each species and classify the leaves as for the presence of stomata. The botanical material was collected in two green areas of Itacoatiara-AM, in Horto Florestal, the leaves of A. pavonina and in the Rubber Tree Forest, the leaves of H. brasiliensis. For each species, leaves from the apex, middle and base were collected, with three samples from each position of the tree from three different individuals. The leaves were preserved in 70% alcohol for further analysis. To determine the stomatal density, the dissociation of the epidermis was done with sodium hypochlorite, stained in safranin and subsequent mounting of slides for analysis under an optical microscope. The stomata had their classification confirmed by means of freehand paradermal cuts. Both species studied presented hypostomatic, homeostomatic leaves with the presence of paracytic stomata. In this study, evidence was found (p= 4,4-46) to affirm that the density of H. brasiliensis differs in the different positions of the tree, and, for the A. pavonina (p= 0,577) no differences were found in relation to stomatal density.