New vectors derived from pUC18 for cloning and thermal-induced expression in Escherichia coli

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorXavier, Mauro Aparecido de Sousa-
Autor(es): dc.creatorKipnis, André-
Autor(es): dc.creatorTorres, Fernando Araripe Gonçalves-
Autor(es): dc.creatorAstolfi Filho, Spartaco-
Data de aceite: dc.date.accessioned2024-07-22T12:11:24Z-
Data de disponibilização: dc.date.available2024-07-22T12:11:24Z-
Data de envio: dc.date.issued2013-08-27-
Data de envio: dc.date.issued2013-08-27-
Data de envio: dc.date.issued2009-10-
Fonte completa do material: dc.identifierhttp://repositorio.unb.br/handle/10482/14041-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S1517-83822009000400007-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/798119-
Descrição: dc.descriptionWe report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.-
Formato: dc.formatapplication/pdf-
Publicador: dc.publisherSociedade Brasileira de Microbiologia-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rightsBrazilian Journal of Microbiology - Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons (Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)). Fonte: http://www.scielo.br/scielo.php?script=sci_serial&pid=1517-8382&lng=pt&nrm=iso. Acesso em: 27 ago. 2013.-
Palavras-chave: dc.subjectEscherichia coli-
Palavras-chave: dc.subjectClonagem molecular-
Palavras-chave: dc.subjectGenética molecular-
Título: dc.titleNew vectors derived from pUC18 for cloning and thermal-induced expression in Escherichia coli-
Aparece nas coleções:Repositório Institucional – UNB

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