Involvement of G proteins and cAMP in the production of chitinolytic enzymes by Trichoderma harzianum

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Autor(es): dc.creatorFirmino, Alexandre A. P.-
Autor(es): dc.creatorUlhoa, Cirano José-
Autor(es): dc.creatorSousa, Marcelo Valle de-
Autor(es): dc.creatorFerreira Filho, Edivaldo Ximenes-
Autor(es): dc.creatorRicart, Carlos André Ornelas-
Data de aceite: dc.date.accessioned2024-07-22T12:09:25Z-
Data de disponibilização: dc.date.available2024-07-22T12:09:25Z-
Data de envio: dc.date.issued2017-12-07-
Data de envio: dc.date.issued2017-12-07-
Data de envio: dc.date.issued2002-
Fonte completa do material: dc.identifierhttp://repositorio.unb.br/handle/10482/25950-
Fonte completa do material: dc.identifierhttps://dx.doi.org/10.1590/S1517-83822002000200015-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/797315-
Descrição: dc.descriptionThe effect of G protein modulators and cyclic AMP (cAMP) on N-acetylglucosaminidase (NAGase) production was investigated during 84 h of growth of a Trichoderma harzianum strain in chitin-containing medium. Caffeine (5 mM), N6--2'-O-dibutyryladenosine 3'5'-cyclic monophosphate sodium salt (dBcAMP) (1 mM) and 3-isobutyl-1-methylxanthine (IBMX) (2 mM) decreased extracellular NAGase activity by 80%, 77% and 37%, respectively. AlCl3/KF (100 µM/10 mM and 200 µM/ 20 mM) decreased the activity by 85% and 95%, respectively. Cholera (10 µ/mL) and pertussis (20 µ/mL) toxins also affected NAGase activity, causing a decrease of approximately 75%. Upon all treatments, protein bands of approximately 73 kDa, 68 kDa and 45 kDa had their signals diminished whilst a 50 kDa band was enhanced only by treatment with cholera and pertussis toxins. N-terminal sequencing analysis identified the 73 kDa and 68 kDa proteins as being T. harzianum NAGase in two different truncated forms whereas the 45 kDa band comprised a T. harzianum endochitinase. The 50 kDa protein showed sequence similarity to Coriolus vesicolor cellobiohydrolase. The above results suggest that a signaling pathway comprising G-proteins, adenylate cyclase and cAMP may be involved in the synthesis of T. harzianum chitinases.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherSociedade Brasileira de Microbiologia-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rightsBrazilian Journal of Microbiology - This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY NC). Fonte: https://www.scielo.br/j/bjm/a/zXzdCBVfcP5XSKPLM6PT9rv/?lang=en#. Acesso em: 10 set. 2021.-
Palavras-chave: dc.subjectTrichoderma harzianum-
Palavras-chave: dc.subjectEnzimas-
Palavras-chave: dc.subjectProteínas-
Título: dc.titleInvolvement of G proteins and cAMP in the production of chitinolytic enzymes by Trichoderma harzianum-
Título: dc.titleEnvolvimento de proteínas G e cAMP na produção de enzimas quitinolíticas por Trichoderma harzianum-
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