Delivery of miRNA-targeted oligonucleotides in the rat striatum by magnetofection with Neuromag®

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorAlmeida, Simoneide Souza Titze de-
Autor(es): dc.creatorHorst, Camila Hillesheim-
Autor(es): dc.creatorSánchez, Cristina Soto-
Autor(es): dc.creatorFernandez, Eduardo-
Autor(es): dc.creatorAlmeida, Ricardo Titze de-
Data de aceite: dc.date.accessioned2021-10-14T18:19:54Z-
Data de disponibilização: dc.date.available2021-10-14T18:19:54Z-
Data de envio: dc.date.issued2020-11-23-
Data de envio: dc.date.issued2020-11-23-
Data de envio: dc.date.issued2018-07-23-
Fonte completa do material: dc.identifierhttps://repositorio.unb.br/handle/10482/39653-
Fonte completa do material: dc.identifierhttps://doi.org/10.3390/molecules23071825-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0001-7286-1676-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/629044-
Descrição: dc.descriptionMicroRNAs (miRNAs) regulate gene expression at posttranscriptional level by triggering RNA interference. In such a sense, aberrant expressions of miRNAs play critical roles in the pathogenesis of many disorders, including Parkinson’s disease (PD). Controlling the level of specific miRNAs in the brain is thus a promising therapeutic strategy for neuroprotection. A fundamental need for miRNA regulation (either replacing or inhibition) is a carrier capable of delivering oligonucleotides into brain cells. This study aimed to examine a polymeric magnetic particle, Neuromag®, for delivery of synthetic miRNA inhibitors in the rat central nervous system. We injected the miRNA inhibitor complexed with Neuromag® into the lateral ventricles next to the striatum, by stereotaxic surgery. Neuromag efficiently delivered oligonucleotides in the striatum and septum areas, as shown by microscopy imaging of fluorescein isothiocyanate (FITC)-labeled oligos in astrocytes and neurons. Transfected oligos showed efficacy concerning miRNA inhibition. Neuromag®-structured miR-134 antimiR (0.36 nmol) caused a significant 0.35 fold decrease of striatal miR-134, as revealed by real-time quantitative polymerase chain reaction (RT-qPCR). In conclusion, the polymeric magnetic particle Neuromag® efficiently delivered functional miRNA inhibitors in brain regions surrounding lateral ventricles, particularly the striatum. This delivery system holds potential as a promising miRNA-based disease-modifying drug and merits further pre-clinical studies using animal models of PD.-
Formato: dc.formatapplication/pdf-
Publicador: dc.publisherMDPI-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rights© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).-
Palavras-chave: dc.subjectMicroRNA-
Palavras-chave: dc.subjectNanopartículas-
Palavras-chave: dc.subjectNeuromag®-
Título: dc.titleDelivery of miRNA-targeted oligonucleotides in the rat striatum by magnetofection with Neuromag®-
Tipo de arquivo: dc.typelivro digital-
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